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Steady-state kinetic parameters for wild type and K315M PAO
| pH 8 | pH 10 | |||||
|---|---|---|---|---|---|---|
| enzyme | kcat/KO2 (mM−1 s−1) | KO2 (mM) | kcat (s−1) | kcat/KO2 (mM−1 s−1) | KO2 (mM) | kcat (s−1) |
| Wild-type | 14 ± 1 | 0.32 ± 0.05 | 4.3 ± 0.2 | 21 ± 3 | 0.99 ± 0.25 | 20.5 ± 2.6 |
| K315M | 1.2 ± 0.1 | 1.0 ± 0.1 | 1.2 ± 0.1 | 0.7 ± 0.1 | 6 ± 6 | 4 ± 4 |
*Conditions: 1 mM N1-acetylspermine, 0.05 mM Tris-HCl (pH 8) or CAPS (pH 10), 20° C
