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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 18;70(11):4666–4675. doi: 10.1158/0008-5472.CAN-09-3970

Figure 3. Regulation of mTOR, raptor and IGF-1R expression by miR99a/miR-100 through their 3′ UTRs.

Figure 3

A, Increasing concentrations of miR-99a and miR-100 pre-miRs (5, 10 and 25 nM) repress expression of Renilla luciferase harboring mTOR, raptor and IGF-1R 3′ UTR sequences in transfected HEK 293T cells. No significant effect was detected for 25 nM control pre-miR. The weak activity of the transfected empty vector (psiCHECK-2) was not affected by cotransfection with any of the pre-miRs. B, Pre-miR-100 (50 nM) efficiently represses activity of luciferase – mTOR, – raptor and – IGF-1R 3′ UTRs, while constructs with deleted miRNA binding sites for each 3′ UTR are not sensitive to repression by pre-miR-100.