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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Tissue Eng Regen Med. 2010 Jun;4(4):247–258. doi: 10.1002/term.228

Figure 3. Expression of scFv EGFRvIII in stably selected hMSC cultured at various temperatures and in HEK cells.

Figure 3

Comparison of surface expression of the scFv EGFRvIII PDGFRtm and hB7-1tm fusion in hMSC cultured at 37°C (A and B) and 32°C (C and D). Expression the scFv EGFRvIII PDGFRtm (E) and hB7-1tm fusion (F) in HEK cells cultured at 37°C 48 hours after transfection. hMSC grown at 37°C for a month show decreased scFvEGFRvIII surface expression (G), which can be “boosted” by transferring cells to 32°C (H) as detected by anti-HA antibodies by flow cytometry.