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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Neurosci Methods. 2010 Apr 10;189(2):197–204. doi: 10.1016/j.jneumeth.2010.04.002

Fig. 2.

Fig. 2

DRG neurons on microislands are predominantly nociceptive. DRG/dorsal horn microisland cultures were fixed and immunostained with antibodies against markers associated with nociceptors. (A) Arrowheads point to DRG neurons positively stained for the nociceptive markers peripherin, TRPV1, and TrkA, whereas arrows in DIC images point to unstained dorsal horn neurons. (B) Shows that most DRG neurons in our microisland culture system are nociceptive with 96% (913/947) expressing substance P (SP), 97% (658/680) CGRP, 96.5% (860/890) TrkA, 94% (851/903) TRPV1, and 98.5% (957/972) peripherin. Cells were counted over 10 different cultures. Scale bar is 50 μm.