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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Neurosci Methods. 2010 Apr 10;189(2):197–204. doi: 10.1016/j.jneumeth.2010.04.002

Fig. 5.

Fig. 5

Paired-recording assay. (A) Typically, a microisland with a DRG neuron and a dorsal horn neuron (Maximum numbers of cell is 3) is selected for patching. DRG neuron is patched in the current-clamp mode and the dorsal horn neuron in the voltage clamp mode. (B) Current steps are given to DRG neurons to assess threshold for action potential and synaptic connection. (C) Sub-threshold stimulation of DRG neurons generally fails to generate action potentials and eEPSCs in the synaptically coupled dorsal horn neuron. (D) Suprathreshold stimulation of DRG neurons generates action potentials, resulting in eEPSCs in the dorsal horn neuron (120 ± 10.02 pA, n=5).