Figure 6. Attenuation of astrocytosis in MPTP-intoxicated mice by nanozyme-loaded BMM.

MPTP-intoxicated C57Bl/6 mice (18 mg/kg) were i.v. injected with PBS (second bar), nanozyme alone (third bar), BMM loaded with nanozyme (5×10⁶ cells/mouse/100µl) (fourth bar), or empty BMM (fifth bar). Mice treated with PBS in lieu of MPTP served as non-intoxicated controls (first bar). Forty-eight hours later animals were sacrificed, and brain tissues were subjected for Western blot with staining for glial fibrillary acidic protein (GFAP). Expression of GFAP shown by A: representative gel; and B: mean densitometry data. Results from N=6 mice per group demonstrating decrease of neuroinflammation in animals treated with catalase nanozyme loaded into the cells, as well as nanozyme alone. No effect on astrocytosis was detected after treatment with empty BMM.