Figure 7. Neuroprotective effect of nanozyme-loaded BMM in an MPTP mouse model of PD.

MPTP-intoxicated C57Bl/6 mice (18 mg/kg) were i.v. injected with PBS (second bar), nanozyme alone (third bar), BMM loaded with nanozyme (5×10⁶ cells/mouse/100µl) (fourth bar), or empty BMM (fifth bar). Healthy non-intoxicated animals were used in a control group (first bar). Seven days later animals were sacrificed, and brain slides were stained for TH-positive nigral dopaminergic neurons. A: Representative images of the same experiment. 10 × magnification, bright field microscopy. B: Results from N=5 animals per group demonstrating significant loss in MPTP treated mice, which is prevented by adoptive transfer of BMM loaded with nanozyme. No significant neuroprotective effect was detected after treatment with nanozyme alone, or empty BMM. Statistical significance is shown by asterisk (p<0.05) compared with ^aPBS; ^bMPTP; ^cMPTP+BMM/nanozyme.