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. 2010 Jun 3;6(6):e1000934. doi: 10.1371/journal.ppat.1000934

Figure 1. p38α in intestinal epithelial cells is required for the immunity to C. rodentium.

Figure 1

A, The level of p38α and phosphorylated-p38α (p-p38α) in isolated IECs from non-infected mice (lane 1), or p38αfl/fl and VillinCre-p38αΔIEC mice infected with C. rodentium for 1 or 2 weeks (lane 2, 4 and 3, 5). RAW264.7 cells, a mouse macrophage cell line, treated with or without LPS for 1 hour were used as controls for p38α phosphorylation (lane 6, 7). The data is representative from two independent experiment sets. B, C. rodentium CFU recovered from colon tissues of individual p38αfl/fl and VillinCre-p38αΔIEC mice at 1, 2, 3, 4 and 5 weeks after inoculation. The data shown are from one experiment, representative of five. The transverse bar is the detection limit. Asterisk, p<0.05. Error bars indicate s.d. (n = 6). N.D. not detected. C, Immunofluorescence staining of C. rodentium in the colon segments by anti-C. rodentium antibodies (red). Nuclei were counterstained with DAPI (blue). Colon segments isolated from a non-infected p38αfl/fl control mouse, and from p38αfl/fl or VillinCre-p38αΔIEC mice at 1 and 2 weeks after infection were used. Scale bar, 100 µm. D, Inflammation detected by hematoxylin/eosin staining of colon segments. The sections are adjacent to those in C. Scale bar, 100 µm. E, The inflammatory cell infiltration into the submucosa in 1 or 2 weeks infected p38αfl/fl or VillinCre-p38αΔIEC mice was evaluated by histological score.