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. 2010 Jun 3;6(6):e1000934. doi: 10.1371/journal.ppat.1000934

Figure 3. Cytokine expressions in the colon of VillinCre-p38αΔIEC mice are impaired after C. rodentium infection.

Figure 3

A–F, ex vivo colon culture ELISA of IFN-γ, IL-17, IL-22, KC, IL-6, and TNF expression in p38αfl/fl or VillinCre-p38αΔIEC mice colons at 1 and 2 weeks after C. rodentium infection. Asterisk, p<0.05; Double asterisk, p>0.05. Error bars indicate s.d. Data are representative of two independent experiments (n = 3). G & H, Expression of IL-17 in the colon mucosa of p38αfl/fl or VillinCre-p38αΔIEC mice at 2 weeks after C. rodentium infection, determined by immunofluorescent staining for CD4 (green) and IL-17 (red), and nuclei were counterstained with DAPI (blue) (G), and by FACS analysis of whole colon cells using anti-CD4-PE and IL-17-APC antibodies (H). The basal levels of CD4 and IL-17 of uninfected p38αfl/fl colon cells are shown in the FACS analysis.