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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Exp Hematol. 2010 Mar 12;38(6):516–525.e4. doi: 10.1016/j.exphem.2010.03.002

Figure 1.

Figure 1

Characterization of donor cells by RT-PCR: Presence of genes pertinent to germ layers and differentiation, and commitment to the pancreatic lineage. hESC was differentiated by co-culture on S17 stromal layer for 17 days (S17/Day 17) or by EB formation for 8 days (EB/day 8). CD34+ cells were isolated from these: (S17/CD34+) and (EB/CD34+). RT-PCR was performed for genes indicated. Lane 1: water; 2: hESC; 3: S17/day 17, 4: S17/CD34+, 5: EB/day 8, 6: EB/CD34+; 7: human fetal pancreas, 8: water, 9: CD34+ from adult BM (BM/CD34+), and 10: human fetal pancreas (22–24 wk).