Table 2.
Animals transplanted with cells differentiated by co-culture of hESC on S17 stroma layer. Transplanted sheep were tested for human C-peptide. Serum was sampled at the specified time point (months) after transplantation. Seven animals tested positive* (see Table 3).
Animal no. | Cell line & passage no. | Differentiated hESC | Cell type injected | Numbers injected | Time sampled (months post-transplantation) |
---|---|---|---|---|---|
1916 | H1 p32 | S17, day 17 | Whole population | 1.5 × 106 | 25 |
*1939 | H1 p59 | S17, day 17 | Whole population | 1.0 × 106 | 31–52 |
*1952 | H1 p67 | S17, day 18 | Whole population | 1.0 × 106 | 24–30 |
*1748 | H1.1 p56 | S17, day 17 | CD34+/CD38− | 140,000 | 38–39 |
1749 | H1.1 p56 | S17, day 17 | CD34+/CD38− | 140,000 | 33 |
2032 | H1 p55 | S17, day 8 | CD34+/CD38− | 200,000 | 19 |
2033 | H1 p55 | S17, day 8 | CD34+/CD38− | 80,000 | 20 |
*2034 | H1 p55 | S17, day 8 | CD34+/CD38− | 80,000 | 20–31 |
1838 | H1 p35 | S17, day 17 | CD34+/Lin− | 95,000 | 30 |
1869 | H1 p43 | S17, day 17 | CD34+/Lin− | 60,000 | 30 |
1871 | H1 p45 | S17, day 17 | CD34+/Lin− | 26,500 | 29 |
*1876 | H1 p44 | S17, day 17 | CD34+/Lin− | 23,500 | 27–29 |
*1885 | H1 p48 | S17, day 17 | CD34+/Lin− | 63,000 | 28–32 |
1886 | H1 p49 | S17, day 17 | CD34+/Lin− | 70,000 | 28 |
*1887 | H1 p49 | S17, day 17 | CD34+/Lin− | 70,000 | 29–55 |