Table 1. Ergosterol feeding relieves growth inhibition by fluconazole.

Treatment	Ab600
Control	1.04±0.01
Control + ergosterol	0.96±0.06
5 µg/ml FLC	1.00±0.02
5 µg/ml FLC + ergosterol	0.94±0.05
25 µg/ml FLC	0.05±0.01
25 µg/ml FLC + ergosterol	0.96±0.01
100 µg/ml FLC	0.04±0.02
100 µg/ml FLC + ergosterol	0.88±0.03

WPY361 (upc2-1 mutant) was grown in YPD containing fluconazole (FLC), ergosterol, or a combination for 30 hours in a 96-well plate at 30°C in quadruplicate. Average of Absorbance values at 600 nm (Ab600) and standard deviations are shown. Ergosterol stock (5 mM in Tween∶ethanol [1∶1]) was added for a final concentration of 50 µM. Equal volume of Tween∶ethanol was added to samples without ergosterol as control.