Figure 5. RIG-I is essential for SeV-induced and sheared poly I:C-induced IRF-3 activation.
A549 were transfected with control (CTRL), RIG-I or Mda5 specific RNAi oligonucleotides as indicated. (A) A549 were further left unstimulated (NS) or infected with SeV (10HAU/106 cells) for various hours post infection (hpi). (B) Cells were further transfected with sheared poly I:C or as control, subjected to the transfection reagent without poly I:C. WCE were resolved by SDS-PAGE and analyzed by immunoblot (IB) using anti-IRF-3-P-Ser396, anti-IRF-3, anti-RIG-I or anti-actin antibodies. Representative immunoblots of three different experiments are shown.