Figure 2. Effect of β-catenin stabilization on isthmic gene expression.
Whole-mount (a–g, h, i,j) and section (g',h',i',j') in situ hybridyzation of E8.5 (a–f'), E9.5 (g–h') and E10.5 (i–j') wt and β-cateninstab embryos for Wnt (a–b') and Fgf8 (c–j') expression. Straight line in g, and h defines position of coronal section shown in g'and h' respectively. Arrows indicate changes in gene expression. Whole mount in situ images show lateral c,d, e,f and dorsal c',d',e',f' views of the same embryos. The scheme represents a model of signal interactions in rhombomere1/midbrain region. β-catenin stimulates expression of Fgf8 but simultaneously inhibits Wnt1 expression itself.