Figure 5. β-catenin inactivation leads to the loss of transcriptional targets of Wnt signalling.

Whole mount and section in situ hybridization of E9.5 (a–d'), E10.5 (e–f') wt and β-catenin^lof embryos using probes against β-catenin (a), Otx2 (b), Fgf8 (c), Wnt1 (d), Ngn2 (e), Aldh1a1 (f). Arrows indicate changes in gene expression. Tamoxifen was injected intraperitoneally into pregnant dams at E8.5, E9.5 followed by embryos isolation 24 hour later.