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. 2010 Jun 3;5(6):e10932. doi: 10.1371/journal.pone.0010932

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative results obtained with this assay are shown for type A isolates known to carry a chromosomal cpe gene (SM101), a plasmid cpe gene with an associated IS1470-like sequence (F4969), or a plasmid cpe gene with an associated IS1151 sequence (F5603). Also shown are representative results for this assay using culture lysates from cpe-positive type C isolates (CN2078, NCTC5388), cpe-positive type D isolates (CN4003) and type E isolates carrying silent cpe sequences (853 and NCIB10748). The migration of molecular size markers is indicated on the left of the blot.