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. 2010 Jun 3;5(6):e10932. doi: 10.1371/journal.pone.0010932

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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An overlapping PCR assay specific for amplification of the type D isolate CN4003 cpe locus region (R1 to R7) was performed using the primer battery shown in Table 4. (A) Map depicting the relationship between ORFs and each reaction in this overlapping PCR battery. * indicates a region with sequence similarity to sequences downstream of cpe in F4969, except for the absence of IS1470-like gene. (B) Products of these reactions using DNA from two representative type D isolates: CN4003 and JGS1902. Numbers at left of each gel indicate migration of size markers in kb.