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. 2010 Jun 3;5(6):e10932. doi: 10.1371/journal.pone.0010932

Figure 8. Detection of potential circular transposition intermediates carrying the cpe gene in type C and D isolates.

Figure 8

(A) Diagram of the cpe locus in type C isolate CN2078 and type D isolate CN4003. (B) PCR amplification of cpe-containing circular intermediates using the primers dcmRseq and cpeMR with CN2078 DNA or primers 1027upNF2 and cpeMR with CN4003 DNA. (C) Diagram derived from sequencing the CN2078 loop product of panel B that was amplified using primers dcmRseq and cpeMR. Black regions of the circle correspond to the amplified product. (D) Diagram derived from sequencing the product from CN4003 loop product of pane B that was amplified using primers 1027upNF2 and cpeMR. Black regions of the circle correspond to the amplified product.