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. 2010 Jun 3;5(6):e10945. doi: 10.1371/journal.pone.0010945

Figure 6. Percentages of abnormal embryos in morpholino treated with or without co-expression of exogenous Atoh8 mRNA.

Figure 6

A: Percentages of abnormal animals with mild or severe phenotypes following the injection of 4 ng or 8 ng morpholinos. MO1-M and MO1-S, percentages of animals with mild and severe phenotypes followed injection with translation blocking morpholino MO1 (total injected n = 169 for 4 ng MO1 and n = 182 for 8 ng MO1), respectively. MO2-M and MO2-S, percentages of animals with mild and severe phenotypes followed injection with splicing blocking morpholino MO2 (total injected n = 173 for 4 ng MO2 and n = 192 for 8 ng MO2), respectively. MIS-MO, morpholino with mismatched sequences (n = 154 for MIS-MO1, and n = 114 for MIS-MO2, 8 ng each). The data were shown by the averages of three independent experiments for each group. B. Overexpression of atoh8 mRNA prevents the severe form of developmental defect in splicing blocking morpholino (MO2) injected embryos (n = 140 for MO2 rescue and n = 192 for MO2 alone, respectively). Two-tail Student t test, * p<0.05, ** p<0.0001.