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. 2010 Jun 3;5(6):e10946. doi: 10.1371/journal.pone.0010946

Figure 6. Foxs and ESRPs promote switching to exon 8 through repressing exon 9 via UGCAUG and ISE/ISS-3.

Figure 6

(A) RT-PCR from HeLa cell transfected the indicated wild-type or cis-mutated reporter vectors and Fox2 and/or ESRP1 expression vectors with or without Fox2 siRNA. The bar graph shows the amount of each splicing product, and is based on calculations from three independent experiments; the mean value for each splice product is show in the respective column, with an error bar showing the SD (standard error). (B) Result of an in vitro splice site recognition assay. The scheme for exon 9 RNA probe shows the position of UGCAUG and ISE/ISS-3. The asterisk shows the probe crosslinked with U1, which binds to the cryptic 5' splice site inside exon 9.