Skip to main content
. Author manuscript; available in PMC: 2010 Jun 3.
Published in final edited form as: Chem Rev. 2009 Mar 11;109(3):839–860. doi: 10.1021/cr800373w

Figure 5.

Figure 5

(a) Electrostatic potential maps of Ras, Raf-RBD, and Ral-RBD. The Ral + 7 mutant contains the mutations M26K/D47K/E54K/D90K, which were predicted by the program PARE to significantly increase the rate constant of association of Ral-RBD to Ras. These four mutations are located outside the Ras-binding site on Ral-RBD. The figure was drawn using the program GRASP, with the contours drawn at 2kBT/e (blue for positive and red for negative). (b) Plot of experimental values of the association rate constants for mutants of Ral-RBD to Ras vs. values calculated using PARE. Adapted with permission from Ref. 14. Copyright 2004 The National Academy of Sciences of the United States of America.