Table 2.
List of primers used in this study
| Primer | Sequence | Description |
|---|---|---|
| For PCR products* | ||
| Forward primers | ||
| 5301 | AAGGCAGGTGAAACAAAACG | 11A cps region 1 PCR amplification |
| 5302 | GAGCTTGGACGAGCTACCAC | 11A cps region 2 PCR amplification |
| 5303 | GGTCAATGGCTTTTTGAGGA | 11A cps region 3 PCR amplification |
| 5304 | TTCGGTGGCAAACCTTTATC | 11A cps region 4 PCR amplification |
| 5305 | GATCCGATTTCTTTGGGTGA | 11A cps region 5 PCR amplification |
| 5306 | GGGAGGCAAAACGTTTGTTA | 11A cps region 6 PCR amplification |
| 5307 | CCCTCGGGCAATGTAGATAA | 11A cps region 7 PCR amplification |
| 5308 | TGCCATCTCGGTTTATTTCC | 11A cps region 8 PCR amplification |
| 5310 | ACCTTTGATTTGCTTCATTATGG | 11A cps region 9 PCR amplification |
| Reverse primers | ||
| 3301 | GTTAAGGTTGGCGCATCAAT | 11A cps region 1 PCR amplification |
| 3302 | TCCTCAAAAAGCCATTGACC | 11A cps region 2 PCR amplification |
| 3303 | CCCAAAGAAATCGGATCAAA | 11A cps region 3 PCR amplification |
| 3304 | TGCGCTGCCTTTCTTTTTAT | 11A cps region 4 PCR amplification |
| 3305 | CCTCAATAATCGCACCACCT | 11A cps region 5 PCR amplification |
| 3306 | GCTTTCATCCCGACAGACAT | 11A cps region 6 PCR amplification |
| 3307 | TATTTGAAAGAGCCGCACCT | 11A cps region 7 PCR amplification |
| 3308 | CTCACAGAAGCACCAGCAAG | 11A cps region 8 PCR amplification |
| 3309 | TTAGCGATCGAACCTGATCC | 11A cps region 9 PCR amplification |
| Additional primers for sequencing | ||
| 5311 | CTTGCTGGTGCTTCTGTGAG | 11A cps region 9 sequencing |
| 5400 | AACGCCGCTACTGTCGTTAT | sp-IS1380 sequencing |
| 5401 | TGACGGACATACGCATGATT | IS1515 sequencing |
| 5409 | GGAGTTGGTAGCCGTCAGTG | Sequencing 11A cps region 1 |
| 5410 | TTTGGCGCTAAGACAGTCTAC | Sequencing 11A cps region 2 |
| 5411 | CTGCTAAAGTTGCTGGTATCC | Sequencing 11A cps region 3 |
| 5415 | CCGTTACGAAGAAATGTCTGTC | Sequencing 11A cps region 7 |
| 3398 | CCCCTGACCTCATGAG | ISSpn5 sequencing |
| 3409 | CTGTACCTACATTACCATTCCCTC | Sequencing 11A cps region 2,3 |
| Additional primers for transformation assayˆ | ||
| 5405 | GGTACCTCGTTAGTTTCCACAGGTGC | 5′ fragment amplification |
| 3405 | TCTAGATCACAGAAGCACCAGCAAGC | 5′ fragment amplification, includes XbaI |
| 5112 | CTAGTCTAGAGTTTGATTTTTAATGG | Janus amplification, includes XbaI |
| 3112 | CGCGGATCCGGGCCCCTTTCCTTATGCTTTTGG | Janus amplification, includes BamH1 |
| 5417 | AGATCTCTTTGTCAACTAACATCTGGAGAG | 3′ fragment amplification, includes BglII |
| 3402 | AACATCCTTCCATTCATCCCCATA | 3′ fragment amplification |
| 3399 | CAAATGTTGGTGGCACAAAG | Complete insert amplification |