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. Author manuscript; available in PMC: 2010 Dec 3.
Published in final edited form as: Oncogene. 2010 Apr 12;29(22):3208–3216. doi: 10.1038/onc.2010.103

Figure 5. Knockdown of Src or Ack1 demonstrates the existence of an additional kinase that induces AR phosphorylation at Tyr-267 after EGF stimulation.

Figure 5

LNCaP cells were transfected with control or Src-specific or Ack1-specific siRNA. After 24 hours, cells were treated with heregulin, Gas6, EGF, IL-6, or bombesin, as indicated, for 60 min. (A) Protein extracts were immunoblotted with the antibody specific for phospho-Y267 AR or total AR. (B) Protein extracts were immunoprecipitated with the AR antibody, then immunoblotted with the antibody specific for phospho-Y534 AR. Protein extracts were also immunoblotted with the antibody specific for Src or Ack1. Blots shown are representative of at least three independent experiments.