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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: Cancer Cell. 2009 Mar 3;15(3):184–194. doi: 10.1016/j.ccr.2009.01.026

Figure 2. DNA damage-induces pRB-dependent activation of proapoptotic gene promoters.

Figure 2

(A) Untreated and doxo treated asynchronously growing T98G cells were assessed for the levels of Caspase 7, p73 and CyclinA2 mRNAs by real time RT-PCR analysis. Results are normalized with GAPDH and shown relative to the levels seen in untreated cells (set to 1). Bars represent the mean of three independent experiments (± SD). (B) T98G cells were untreated or treated with 2μM doxo for 16 hours and the binding of pRB, E2F1, RNA Pol II and HDAC1 to the Caspase7, p73 and Cyclin A2 gene promoters determined by ChIP. (C) AcH4 re-ChIP analysis of the pRB ChIP shows that pRB is bound to the transcriptionally active Caspase7 gene promoter following doxo treatment.