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DAF-2T is detected with LC-MS in E-cluster homogenate after incubation with DAF-2 DA. Upper panel: Region of base peak chromatogram showing the retention time of DAF-2T. Middle panel: Region of the full MS spectrum showing the experimental m/z (M+H)+ of 374.1, indicative of DAF-2T. Lower panel: MS/MS spectrum confirming the identity of DAF-2T signal.
