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(a) Bright-field and (b) fluorescence from neurons in buccal ganglia incubated in ASW, (c) 1 mM l-arginine, and (d) 0.1 mM NOS inhibitor l-NAME, all treatments were done for 3 h before DAF-2 DA intracellular loading. All instrument settings for the images are the same. Scale bar = 50 μm.
