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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: Front Biosci (Elite Ed). 2010 Jun 1;2:1154–1163. doi: 10.2741/e175

Figure 7.

Figure 7

EGR1 binds to the putative EGR1 binding site in the FAP promoter. [γ-32P] ATP-labeled oligonucleotides covering −225 to −185 region of FAP promoter fragment was incubated with recombinant EGR1 protein (“Control”). Competition experiment was performed by pre-incubating EGR1 protein with a 100-fold excess of unlabeled consensus (“cold WT probe”) or EGR1 mutant oligos (“cold mutant probe”) prior to the addition of the 32P-labeled oligo. EGR1 antibody was used for supershift analysis. Arrows point at the DNA/protein complex of EGR1 and supershift band identified by the EGR1 antibody.