Figure 2.
(A) Fluorescence polarization-based competitive binding curves of compound 11 for Bcl-XL (red squares), Bcl-2 (blue dots), Bfl-1 (dark up triangle) and Mcl-1 (green up triangle) (B) Inhibition of cell growth by compound 4 (red dots), 11 (blue dots), 12 (green dots), 13 (yellow dots) and 14 (orange dots) in the PC-3 human prostate cancer cell line. Cells were treated for 3 days and cell viability was evaluated using ATP-LITE assay. (C) Inhibition of cell growth by compound 4 (deep blue dots), 11 (red square), 12 (green down triangle), 13 (light blue diamonds) and 14 (grey up triangle) in the H460 human lung cancer cell line. Cells were treated for 3 days and cell viability was evaluated using ATP-LITE assay. (D) Inhibition of cell growth by compound 11 (dark square) in the human BP3 cell line. Apoptosis was monitored by Annexin V-FITC assays.