Figure 5.
Characterization of compounds 4 and 11 in tumor xenografts model. Tumor xenografts from M2182 cells were established in athymic nude mice on the left and right flanks. After establishing visible tumors of ~75-mm3, DMSO or compounds (4 or 11) were given (i.p.) every two days (total of nine injections). A minimum of five animals was used per experimental group. For in vivo imaging of tumors the mice were anesthetized and injected i.p. with 150 mg/kg luciferin and light emitted from each tumor was determined in a xenogen system with CCD camera using an integration time of 1 min. Luminescence measurements were made using Living Image software (version 2.50.1; Xenogen). At the end of the experiment, the animals were sacrificed, and the tumors were removed and photographed. (A) Left flank tumor treated with compound 4 at dose of 10 mg/kg and 25 mg/kg, respectively. (B) Right flank tumor treated with compound 4 at dose of 10 mg/kg and 25 mg/kg, respectively. (C) Left flank tumor treated with compound 11 at dose of 1, 3 and 5 mg/kg, respectively. (D) Right flank tumor treated with compound 11 at doses of 1, 3 and 5 mg/kg, respectively.