Table 1. Ct Values from a Representative qRT-PCR Assay for Cytokine Effect on MUC1 and MUC 16 Expression.
| I Hour | MUC1 | MUC16 | GAPDH |
|---|---|---|---|
| IFN-γ 1 | 33.21 | 29.47 | 23.50 |
| IFN-γ 2 | 31.92 | 30.25 | 22.65 |
| IFN-γ 3 | 33.34 | 29.91 | 24.34 |
| VC 1 | 32.18 | 28.62 | 22.55 |
| VC 2 | 29.62 | 26.43 | 20.00 |
| VC 3 | 29.32 | 25.97 | 20.63 |
| 6 Hours | MUC1 | MUC16 | GAPDH |
|---|---|---|---|
| IFN-γ 1 | 31.54 | 29.67 | 26.28 |
| IFN-γ 2 | 30.94 | 28.84 | 25.84 |
| IFN-γ 3 | 31.86 | 29.16 | 25.55 |
| VC 1 | 31.61 | 28.60 | 23.92 |
| VC 2 | 32.30 | 29.18 | 24.43 |
| VC 3 | 32.47 | 28.95 | 24.49 |
| 24 Hours | MUC1 | MUC16 | GAPDH |
|---|---|---|---|
| IFN-γ 1 | 26.38 | 26.12 | 21.97 |
| IFN-γ 2 | 27.35 | 26.42 | 21.93 |
| IFN-γ 3 | 26.20 | 25.61 | 21.42 |
| VC 1 | 28.79 | 27.88 | 22.84 |
| VC 2 | 31.03 | 27.66 | 22.12 |
| VC 3 | 34.12 | 30.49 | 26.49 |
| 48 Hours | MUC1 | MUC16 | GAPDH |
|---|---|---|---|
| IFN-γ 1 | 28.60 | 27.00 | 25.83 |
| IFN-γ 2 | 26.41 | 25.11 | 23.62 |
| IFN-γ 3 | 37.22 | 33.33 | 34.09 |
| VC 1 | 31.29 | 26.97 | 24.10 |
| VC 2 | 31.86 | 27.88 | 24.53 |
| VC 3 | 33.38 | 29.92 | 25.36 |
IFN-γ: Interferon-γ Treatment
VC: Vehicle Control