Figure 2.

Preferential orientation for DNA cleavage by FPV resolvase on Junction 1^S. DNA oligonucleotides were 5'-end labeled with ³²P on the strands indicated above the gels. Resolvase enzymes studied are marked above the gel lanes. Fowlpox resolvase, Cce I protein or T7 endonuclease I (New England Biolabs, Beverley, MA) were added to a solution containing HJ1^S DNA (final concentrations; 10 nM resolvase, 10 nM Cce I, 2U of T7 endonuclease and 2nM HJ1^S DNA substrate). Reaction mixtures were incubated at 37 °C for 30 min. Products of the reactions were separated on urea-containing 10% polyacrylamide gels by electrophoresis in 1X TBE buffer and visualized by autoradiography.