Table 2.
Rate analysis for the metal rescue experiment.
Reactions were started by addition of metal cofactor. Substrate and product bands from native-PAGE analysis of DNA cleavage reactions were quantified and percent product was calculated. Rates were calculated using a single exponential decay model. From 15–18 points were used to determine each rate.
| Enzyme | Rate of Cleavage (pM/sec) | Ratio of rates 0.5 mM Mn/ 15 mM Mg | |
|---|---|---|---|
| 0.5 mM Mn | 15 mM Mg | ||
| D7C | 0.7543±0.0453 | 0.0428±0.0192 | 17.6 |
| E60C | 0.2072±0.0301 | 0.0990±0.0276 | 2.1 |
| D132C | 0.5117±0.0853 | 0.2135±0.0354 | 2.4 |
| D135C | 5.9166±0.3227 | 0.2541±0.0510 | 23.2 |