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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: J Exp Zool B Mol Dev Evol. 2009 Jun 15;312B(4):292–308. doi: 10.1002/jez.b.21266

Fig. 4.

Fig. 4

Dental epithelium on frontal sections of mandible at embryonic day 13.5. In each wild-type (WT) and mutant (Spry2−/−) embryo, the large posterior diastemal bud (R2) and molar epithelium (M) is presented on histological sections stained with hematoxylin–eosin prestained with alcian blue (H&E) and after Shh whole mount in situ hybridization. At the enamel knot area at the tip of the R2 bud (arrowhead) in WT and Spry2−/− embryos, Shh is expressed, whereas it is absent in the molar epithelium. Note the increased Shh expression in the mutant R2 bud.