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. 2010 Apr 13;9(6):3200–3217. doi: 10.1021/pr100068p

Figure 4.

Figure 4

ChIP experiments showing direct binding of VjbR on several promoter regions. (A) Detection of several virB1-virB2 regions. (B) Detection of BMEII0734, BMEII0590, BMEI0030, BMEI1305, BMEI0668 and BMEI1007 promoter regions. All ChIP were performed with a C-terminal Flag-tagged VjbR-HTH protein expressed from a high copy plasmid (pSB502). The y-axis represents the ratio of immunoprecipited product (IP) versus input (IN) (%IP/IN). White columns represent IP from control strain (ΔvjbR, empty plasmid), gray columns represent IP from (ΔvjbR, pSB502). Error bars represent standard deviation from three independent experiments.