Table 4. Validation of Some Targets by qRT-PCR and Analysis of C₁₂−HSL Effect^a.

	babR	vjbR	dnaK	virB2	groEL	groES	BMEI0433	BMEI0668	BMEII0625
wt + ACN	1.0	1.0	1.0	1.0	1.0	1.0	1.0	1.0	1.0
wt + C12−HSL	2.6	0.5	1.6	0.2	2.0	2.2	2.1	4.5	1.8
ΔbabR + ACN	0	1.7	1.9	1.9	3.1	2.7	1.1	0.6	0.8
ΔbabR + C12−HSL	0	0.3	1.8	0.1	4.0	3.3	0.8	3.5	2.2
ΔvjbR + ACN	0.7	0	0.9	0.1	0.1	0.1	2.9	4.5	3.8
ΔvjbR + C12−HSL	1.5	0	1.2	0.1	0.2	0.2	4.4	9.7	4.9

^aComparison of fold change ratios for mRNA from wt, ΔbabR and ΔvjbR strains with or without C₁₂−HSL. RNA was extracted at an equivalent OD600 for the transcriptomic and the qRT-PCR experiments. ACN: Acetonitrile: C₁₂−HSL solvent. C₁₂−HSL: dodecanoyl-L-homoserine lactone (added to the culture media at a final concentration of 5 mM). We considered that gene expression is different between wt and mutant strain when the ratio is >1.3 or <0.7.