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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2010 Mar 25;80(2):188–196. doi: 10.1016/j.bcp.2010.03.020

Figure 4.

Figure 4

Effect of the A3 receptor antagonist MRS1191 on the protection by IB-MECA against H2O2-induced cell damage. HL-1 cells were pretreated with MRS1191 (10 μM), an antagonist of the A3 receptor, 1 h before treatment with IB-MECA (30 μM). One h after the addition of the agonist, H2O2 (750 μM) was incubated with the cells for 4 h. 100 μl of media was then added to 100 μl of the LDH measuring solution provided with the kit and incubated for 10 min. The results were analyzed with a microplate reader. Data shown are mean ± SD from three independent experiments in triplicate. Groups labeled * are significantly different from H2O2 control (P < 0.05).