Figure 7.

TLX–Wnt/β-catenin signalling regulates neural stem cell population in the adult brain. (a) Lentiviral tranduction of active β-catenin increases the numbers of GFAP⁺ B cells in the SVZ of wild-type (WT) brains. Brains were injected with control GFP lentivirus (GFP) or β-catenin ΔN90–GFP virus (β-catenin–GFP). Scale bar, 10 μm. (b) Quantification of the percentage of GFAP⁺GFP⁺ cells out of total GFP⁺ cells in control GFP (C) and β-catenin–GFP (β-cat.)-transduced SVZ. Data are represented as means ± s.d.; asterisk, P < 0.05 by Student’s t-test, n = 10. (c) Decrease in number of BrdU-label-retaining cells in the SVZ of Wnt7a^−/− brains. BrdU-label-retaining cells were labelled in green. S100β staining (red) is included to show the structure of the SVZ. Scale bar, 20 μm. (d) Quantification of the BrdU-label-retaining (BrdU⁺) cells in the SVZ of WT and Wnt7a^−/− (Wnt^−/−) brains. The fold change in BrdU⁺ cells in the SVZ of WT mice is calculated by dividing the number of BrdU⁺ cells in the SVZ of WT mice by the number of BrdU⁺ cells in the SVZ of Wnt7a^−/− SVZ. The fold change in Wnt7a^−/− SVZ is expressed as 1. Data are represented as means ± s.d.; asterisk, P < 0.001 by Student’s t-test, n = 8 for WT brains and n = 12 for Wnt7a^−/− brains. (e) Decrease in number of BrdU-label-retaining cells in the SVZ of TLX^−/− brains. BrdU labelling is shown in green and S100β in red. Scale bar, 20 μm. (f) Quantification of BrdU-label-retaining (BrdU⁺) cells in the SVZ of WT and TLX^−/− brains. The fold change in BrdU⁺ cells in the SVZ is calculated in the same way as in (d). Data are represented as means ± s.d.; asterisk, P < 0.001 by Student’s t-test, n = 5 for both wild-type and TLX^−/− brains.