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. Author manuscript; available in PMC: 2010 Sep 26.
Published in final edited form as: Mol Cell. 2010 Mar 26;37(6):834–842. doi: 10.1016/j.molcel.2010.01.037

Figure 2. Measurement of the thermodynamic constants for nucleosome formation.

Figure 2

The experimental design for each reaction is shown above each panel, using the symbols described in Fig. 1. Fluorescent labels are indicated by asterisks; FRET is indicated by a red arrow. Closed squares are 601 DNA or (H3–H4)2 tetramer-bound 601 sequence (601-tetrasomes); closed triangles are 5S sequence DNA or (H3–H4)2 tetramer-bound 5S DNA (5S tetrasomes). (A) Normalized fluorescence as a function of DNA binding to H3–H4. (B) The change in FRET between Nap1 and H2B as a function of tetrasome ((H3–H4)2-DNA). (C) The change in FRET between Nap1 and H2B as a function of (H3–H4)2. The Nap1 concentration is kept at the Kd for the Nap1-H2A–H2B dimer complex (K2, Table 1).