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Micro-patterned Tol-BMP-ST supported spatially restricted differentiation of calvarial-derived cells. Tol-BMP-St was patterned onto gold surfaces using a soft lithography approach to create 150 μm diameter circles of scaffold protein. Cells were cultured on these surfaces for 14 days. (a) Cell distribution was assessed by DAPI staining for nuclear DNA and (b) cell differentiation was investigated by alkaline phosphatase staining (blue).
