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SPR data of the Tol, Tol-Osteopontin (Tol-OP) and Tol-BMP proteins binding to a gold surface in SPR analysis (BIAcore).
| Protein | rU increase | Mr of protein | moles/mm2 | Molecules/mm2 |
|---|---|---|---|---|
| Tol | 1,526.53 | 1,4874.4 | 1.03 × 10-13 | 6.4 × 1010 |
| Tol-Op | 4,381.15 | 18,407.3 | 2.38 × 10-13 | 14.8 × 1010 |
| Tol-BMP | 3,162.26 | 17,894.8 | 1.77 × 10-13 | 11.0 × 1010 |
The proteins were added at a concentration of 5 mM in a total volume of 30 μl over a period of 15 minutes.
