Skip to main content
. 2010 Apr 26;8:41. doi: 10.1186/1479-5876-8-41

Table 4.

Formation of neutralizing anti-Ab-02 antibodies (log Titer) after a single 10 mg/kg IV dosage of Ab-02 to cynomolgus monkeys

TIME
(DAYS)
ANIMAL 4 ANIMAL 5 ANIMAL 6
Pre-dose Negative Negative Negative
15 Negative Negative Negative
22 4.12 2.7 2.2
36 2.2 2.7 2.7

Formation of neutralizing anti-Ab-02 was assessed using a flow cytometric assay, in which serum samples from Ab-02 dosed monkeys were tested for inhibition (relative to pre-dose) of anti-IL-21R-biotin binding to TF-1 cells transfected with human IL-21R. Negative samples had no inhibition at the minimum required dilution of 1:6 and had a log titer < 0.78.