Table 1. Sequences of the primers and TaqMan® probes.
| Gene (GenBank accession number) | Oligonucleotide sequence (5′-3′) |
| Prdm16 (NM_027504) | |
| Forward | GATGGGAGATGCTGACGGATAC |
| Reverse | CTCGCTACCCAAGTCTTCAGAGAT |
| TaqMan® Probe | FAM- CATCCCAGGAGAGCTGATCAAAAAGC-TAMRA |
| Rip140 (NM_173440) | |
| Forward | TCAGCTTCCTTTCCCACATAGC |
| Reverse | TCATCTTTCGTTGCTCACCAAA |
| TaqMan® Probe | FAM-AGGCTCAGGCTGAGGCAGACGATACT-TAMRA |
| Bmp7 (NM_07557) | |
| Forward | CAAGACGCCAAAGAACCAAGAG |
| Reverse | GGTCTCGGAAGCTGACGTACAG |
| TaqMan® Probe | FAM-ATGGCCAGTGTGGCAGAAAACAGCA-TAMRA |
| Sirt-1 (NM_019812) | |
| Forward | AGCAGGTTGCAGGAATCCAA |
| Reverse | CACGAACAGCTTCACAATCAACTT |
| TaqMan® Probe | FAM-CCTTCAGTGTCATGGTTCCTTTG-TAMRA |
| Sirt-3 (NM_001127351) | |
| Forward | CTGACTTCGCTTTGGCAGATCT |
| Reverse | CCCCACCAAGTCTCGATTGAT |
| TaqMan® Probe | FAM-CTGGAGGTGGAGCCTTTTGCCAGCT-TAMRA |
| Pgc-1α (NM_008904) | |
| Forward | TGAACGCACCTTAAGTGTGGAA |
| Reverse | GGGTTATCTTGGTTGGCTTTATGA |
| TaqMan® Probe | FAM-ATCGCAGGCCTAACTCCACCCACCA -TAMRA |
| Ucp1 (NM_009463) | |
| Forward | CGATGTCCATGTACACCAAGGA |
| Reverse | ACCCGAGTCGCAGAAAAGAAG |
| TaqMan® Probe | FAM-ACCGACGGCCTTTTTCAAAGGGTTTG-TAMRA |
| Ucp3 (NM_001030877) | |
| Forward | GACCTACGACATCATCAAGGAGAAGT |
| Reverse | CTCCAAAGGCAGAGACAAAGTGA |
| TaqMan® Probe | FAM-6TCTCACCTGTTTACTGACAACTTCCC-TAMRA |
Prdm16, PR domain containing 16; Rip140, receptor-interacting protein 140; Bmp7, bone morphogenetic protein 7; Sirt-1, sirtuin-1, Sirt-3, sirtuin-3; Pgc-1α, peroxisome proliferative activated receptor γ coactivator 1 α; Ucp-1, uncoupling protein 1, Ucp-3, uncoupling protein 3.