Table 1. Sequences of the primers and TaqMan® probes.
Gene (GenBank accession number) | Oligonucleotide sequence (5′-3′) |
Prdm16 (NM_027504) | |
Forward | GATGGGAGATGCTGACGGATAC |
Reverse | CTCGCTACCCAAGTCTTCAGAGAT |
TaqMan® Probe | FAM- CATCCCAGGAGAGCTGATCAAAAAGC-TAMRA |
Rip140 (NM_173440) | |
Forward | TCAGCTTCCTTTCCCACATAGC |
Reverse | TCATCTTTCGTTGCTCACCAAA |
TaqMan® Probe | FAM-AGGCTCAGGCTGAGGCAGACGATACT-TAMRA |
Bmp7 (NM_07557) | |
Forward | CAAGACGCCAAAGAACCAAGAG |
Reverse | GGTCTCGGAAGCTGACGTACAG |
TaqMan® Probe | FAM-ATGGCCAGTGTGGCAGAAAACAGCA-TAMRA |
Sirt-1 (NM_019812) | |
Forward | AGCAGGTTGCAGGAATCCAA |
Reverse | CACGAACAGCTTCACAATCAACTT |
TaqMan® Probe | FAM-CCTTCAGTGTCATGGTTCCTTTG-TAMRA |
Sirt-3 (NM_001127351) | |
Forward | CTGACTTCGCTTTGGCAGATCT |
Reverse | CCCCACCAAGTCTCGATTGAT |
TaqMan® Probe | FAM-CTGGAGGTGGAGCCTTTTGCCAGCT-TAMRA |
Pgc-1α (NM_008904) | |
Forward | TGAACGCACCTTAAGTGTGGAA |
Reverse | GGGTTATCTTGGTTGGCTTTATGA |
TaqMan® Probe | FAM-ATCGCAGGCCTAACTCCACCCACCA -TAMRA |
Ucp1 (NM_009463) | |
Forward | CGATGTCCATGTACACCAAGGA |
Reverse | ACCCGAGTCGCAGAAAAGAAG |
TaqMan® Probe | FAM-ACCGACGGCCTTTTTCAAAGGGTTTG-TAMRA |
Ucp3 (NM_001030877) | |
Forward | GACCTACGACATCATCAAGGAGAAGT |
Reverse | CTCCAAAGGCAGAGACAAAGTGA |
TaqMan® Probe | FAM-6TCTCACCTGTTTACTGACAACTTCCC-TAMRA |
Prdm16, PR domain containing 16; Rip140, receptor-interacting protein 140; Bmp7, bone morphogenetic protein 7; Sirt-1, sirtuin-1, Sirt-3, sirtuin-3; Pgc-1α, peroxisome proliferative activated receptor γ coactivator 1 α; Ucp-1, uncoupling protein 1, Ucp-3, uncoupling protein 3.