Table 2. Metabolic characteristics of 12-week-old experimental animals.

	Wild type	iNOS−/−	ob/ob	DBKO
Glucose (mg/dl) a	75±4	79±2	105±7	99±4
Glycerol (mg/dl) a	0.022±0.002	0.023±0.003	0.034±0.001	0.032±0.002
FFA (mmol/l) a, b	0.70±0.06	0.58±0.03	1.13±0.10	0.85±0.04
TG (mg/dl) a	70±4	77±4	97±8	96±7
Cholesterol (mg/dl) a, b	83±7	71±4	177±15	158±11
Insulin (ng/ml) a, c	0.39±0.01	0.33±0.04d	11.20±1.54e	8.75±0.81e,f
HOMA a	1.78±0.22	1.55±0.18	79.45±13.93	47.27±3.14
Adiponectin (µg/ml) a	23±3	26±3	14±2	20±4

Data are means ± SEM of 8–10 animals. P values obtained by two-way ANOVA are shown. One way ANOVA followed by Tukey's post hoc tests were applied for variables with interaction between factors.

^aeffect of the absence of the ob gene (p<0.01);

^beffect of the absence of the iNOS gene (p<0.01);

^cinteraction between factors (p<0.05);

^d p<0.05 vs wild type;

^e p<0.001 vs wild type;

^f p = 0.071 vs ob/ob. FFA: free fatty acids, HOMA: homeostasis model assessment, TG: triglycerides. DBKO: double knockout mice simultaneously lacking the ob and iNOS genes.