Table 2. Metabolic characteristics of 12-week-old experimental animals.
Wild type | iNOS−/− | ob/ob | DBKO | |
Glucose (mg/dl) a | 75±4 | 79±2 | 105±7 | 99±4 |
Glycerol (mg/dl) a | 0.022±0.002 | 0.023±0.003 | 0.034±0.001 | 0.032±0.002 |
FFA (mmol/l) a, b | 0.70±0.06 | 0.58±0.03 | 1.13±0.10 | 0.85±0.04 |
TG (mg/dl) a | 70±4 | 77±4 | 97±8 | 96±7 |
Cholesterol (mg/dl) a, b | 83±7 | 71±4 | 177±15 | 158±11 |
Insulin (ng/ml) a, c | 0.39±0.01 | 0.33±0.04d | 11.20±1.54e | 8.75±0.81e,f |
HOMA a | 1.78±0.22 | 1.55±0.18 | 79.45±13.93 | 47.27±3.14 |
Adiponectin (µg/ml) a | 23±3 | 26±3 | 14±2 | 20±4 |
Data are means ± SEM of 8–10 animals. P values obtained by two-way ANOVA are shown. One way ANOVA followed by Tukey's post hoc tests were applied for variables with interaction between factors.
effect of the absence of the ob gene (p<0.01);
effect of the absence of the iNOS gene (p<0.01);
interaction between factors (p<0.05);
p<0.05 vs wild type;
p<0.001 vs wild type;
p = 0.071 vs ob/ob. FFA: free fatty acids, HOMA: homeostasis model assessment, TG: triglycerides. DBKO: double knockout mice simultaneously lacking the ob and iNOS genes.