Table 2. In vivo phosphorylation stoichiometry (% of phosphorylation) for the PSII core proteins from the leaves of wild type, stn7, stn8 and stn7stn8 mutant plants harvested during day or night.

	Wild type		stn8
	12h Dark	4h Light	12h Dark	4h Light
Protein	% of phosphorylation		% of phosphorylation
P-CP43	49±12	34±13	16±8	13±7
P-D1	27±12	36±15	12±9	14±8
P-D2	33±11	42±12	10±6	10±4
P-PsbH	29±9	24±12	11±8	9±8
PP-PsbH	N.D.	54±14	N.D.	N.D.

N.D. - not determined.

*The PsbH (AtQTVEDSSR) and D2 (Ac-tIALGK) phosphopeptide signals were detected in the samples from stn7stn8 only in one or two out of three different experiments.

The phosphorylation state of each protein was determined from signal intensities of peptide and phosphopeptide ion pairs corrected using corresponding flyability ratios from the Table 1. Data are the average from three experiments in each condition and three LC-MS runs for every experiment.