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. 2010 May 7;9:101. doi: 10.1186/1476-4598-9-101

Figure 1.

Figure 1

OPN-induces ICAM-1 expression in breast cancer cells. A and C. MCF-7 or MDA-MB-468 cells were treated with 0.5 μM OPN in serum free medium for 0-24 h. B and D. In a separate experiments, MCF-7 or MDA-MB-468 cells were treated with various concentrations of OPN (0-5.0 μM) for 24 h. Cell lysates containing equal amount of total proteins were analyzed by western blot using anti-ICAM-1 antibody. Actin was used as loading control.