Figure 4.

mTOR down reguates OPN-induced AP-1 activation. A and B. MCF-7 cells were treated with 0.5 μM OPN for 0-240 min or transiently transfected with wild type or rapamycin resistant mTOR and then treated with OPN. The non-transfected or transfected cells were treated with rapamycin alone or along with OPN. Nuclear extracts were prepared and analyzed by EMSA. C. MCF-7 cells were transiently transfected with wild type or rapamycin resistant mTOR along with pAP-1-Luc. The non-transfected or transfected cells were treated with rapamycin alone or along with OPN. The transfection efficiency was normalized by Renilla and fold changes in luciferase activity with respect to control were calculated, and mean ± S.D. of triplicate determinations are plotted. The values were also analyzed by One Way ANOVA (*, p < 0.05; **, p < 0.05).