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. 2010 May 7;9:101. doi: 10.1186/1476-4598-9-101

Figure 6.

Figure 6

OPN induces phosphorylation of p70S6 kinase at Thr-421/Ser-424, but not at Thr-389 and Ser-371 and has no effect on mTOR phosphorylation. A-D. MCF-7 cells were either treated with 0.5 μM OPN for 0-120 min or pretreated with 20 nM rapamycin for 1 h and then treated with 0.5 μM OPN for 10 min. Cell lysates containing equal amount of total proteins were resolved by SDS-PAGE and immunoblotted with anti-p-mTOR or anti-p-p70S6 kinase (Thr-421/Ser-424, Ser-371 or Thr-389) antibody. These blots were reprobed with anti-mTOR or anti-p70S6 kinase antibody.