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Hypothetical Illumina GA flow cell with mRNA isolated from subjects within seven different treatment groups 
and loaded into individual lanes (e.g., the mRNA from the subject within treatment group 1 is sequenced in lane 1). As a control, a 
genomic sample is often loaded into lane 5. The bacteriophage 
genome is known exactly and can be used to recalibrate the quality scoring of sequencing reads from other lanes (Bentley et al. 2008).
