Figure 3.—

Full-length Yng1 binds unmodified histone H3 tails independently of the PHD finger. (A and B) Histone tail peptide-binding assays were performed with the indicated immobilized peptides and purified GST fusion proteins. Shown are αGST Western blots of precipitated material. Input lanes contain 10% of the proteins used for the pulldown. (C) A gcn5Δ yng1Δ strain expressing GCN5 from a URA3-based plasmid was transformed with the indicated plasmids, plated on synthetic complete medium with and without 5-FOA, and incubated at 30° for 3 days.