Figure 4.—

The amino terminus of Yng1 is required for binding of unmodified histone H3 tails. (A) Histone tail peptide binding assays were performed with the indicated immobilized peptides and purified GST fusion proteins. Shown are αGST Western blots of precipitated material. Input lanes contain 10% of the proteins used for the pulldown. (B) Tenfold serial dilutions of a wild-type yeast strain containing the indicated low-copy plasmids were plated on synthetic uracil drop-out medium containing either dextrose or galactose as a carbon source and incubated at 30° for 3 days.